Journal: Antioxidants

Article Title: Weaning Mice and Adult Mice Exhibit Differential Carbon Tetrachloride-Induced Acute Hepatotoxicity

doi: 10.3390/antiox9030201

Figure Lengend Snippet: Hepatic CYP2E1 and CYP3A levels in the weaning mice and adult mice without carbon tetrachloride (CCl 4 ) treatment. Hepatic CYPs were detected by immunoblotting and GAPDH was used as the loading control. The relative protein amounts are presented as mean ± standard deviation (SD). *, *** Significantly different from the corresponding weaning mice (Student’s t -test, p < 0.05 and p < 0.001, respectively).

Article Snippet: Next, the membrane was incubated with the following antibodies for 12 h: anti-cysteine dioxygenase (CDO), anti-glutamate cysteine ligase catalytic subunit (GCLC), anti-GSH peroxidase (GPx), anti-GSH reductase (GR), anti-nitrotyrosine (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-CYP2E1, anti-CYP3A (Detroit R&D, Detroit, MI, USA), anti-c-Jun N terminal kinase (JNK), anti-phospho-JNK, anti-extracellular regulated protein kinase (Erk), anti-phospho-Erk, anti-p38, anti-phospho-p38, anti-caspase-3, anti-cleaved caspase-3, anti-caspase-7, anti-cleaved caspase-7, anti-caspase-9, anti-cleaved caspase-9, anti-caspase-12, anti-cleaved caspase-12, anti-poly (ADP-ribose) polymerase (PARP) (Cell Signaling Technology, Danvers, MA, USA), anti-GSH S-transferase-α (GST-α), anti-GST-µ, and anti-GST-π (Detroit R&D).

Techniques: Western Blot, Standard Deviation

Journal: Antioxidants

Article Title: Weaning Mice and Adult Mice Exhibit Differential Carbon Tetrachloride-Induced Acute Hepatotoxicity

doi: 10.3390/antiox9030201

Figure Lengend Snippet: Summary and hypothetical mechanisms of age-related susceptibility to carbon tetrachloride (CCl 4 ) hepatotoxicity in weaning mice and adult mice. Higher metabolic rate of CCl 4 by hepatic CYP2E1 and CYP3A in weaning mice can cause excessive oxidative stress via reactive oxygen species (ROS) generation and glutathione (GSH) depletion, which can lead to significant liver injury. However, adult mice exhibit less CYP levels and high levels of GST-π and GR in their livers. This can contribute to resistance to CCl 4 hepatotoxicity.

Article Snippet: Next, the membrane was incubated with the following antibodies for 12 h: anti-cysteine dioxygenase (CDO), anti-glutamate cysteine ligase catalytic subunit (GCLC), anti-GSH peroxidase (GPx), anti-GSH reductase (GR), anti-nitrotyrosine (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-CYP2E1, anti-CYP3A (Detroit R&D, Detroit, MI, USA), anti-c-Jun N terminal kinase (JNK), anti-phospho-JNK, anti-extracellular regulated protein kinase (Erk), anti-phospho-Erk, anti-p38, anti-phospho-p38, anti-caspase-3, anti-cleaved caspase-3, anti-caspase-7, anti-cleaved caspase-7, anti-caspase-9, anti-cleaved caspase-9, anti-caspase-12, anti-cleaved caspase-12, anti-poly (ADP-ribose) polymerase (PARP) (Cell Signaling Technology, Danvers, MA, USA), anti-GSH S-transferase-α (GST-α), anti-GST-µ, and anti-GST-π (Detroit R&D).

Techniques: